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Image Search Results
Journal: Clinical and Vaccine Immunology : CVI
Article Title: Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs
doi: 10.1128/CVI.00519-13
Figure Lengend Snippet: Comparison of IFN-γ responses to liquid and lyophilized antigen preparations from control (n = 5) as well as M. bovis Ravenel- (n = 5) and M. bovis 95-1315-infected (n = 5) animals. (a) Response kinetics: PPDb (Lelystad) minus no stimulation. For control animals, the responses to liquid and lyophilized antigens were equivalent; thus, the lines on the graphs are overlapping. Responses to PPDb detected prior to challenge in all groups (including controls) were likely due to cross-reactive responses elicited by nontuberculous Mycobacteria spp. (b) Liquid and lyophilized antigen preparations pooled over time. Responses to pokeweed mitogen (PWM) are included as a positive controls for cell viability. (c) Response kinetics: PPDb minus PPDa (Lelystad PPDs).
Article Snippet: PPDs and peptide cocktails were also used for comparison of a
Techniques: Infection
Journal: Clinical and Vaccine Immunology : CVI
Article Title: Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs
doi: 10.1128/CVI.00519-13
Figure Lengend Snippet: Comparison of IFN-γ responses to PC-HP and PC-EC in control (n = 5) and M. bovis Ravenel- (n = 5) and M. bovis 95-1315-infected animals. (a) Responses over time and (b) responses pooled over time. **, responses differ between groups (P < 0.01).
Article Snippet: PPDs and peptide cocktails were also used for comparison of a
Techniques: Infection
Journal: Clinical and Vaccine Immunology : CVI
Article Title: Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs
doi: 10.1128/CVI.00519-13
Figure Lengend Snippet: IFN-γ responses to Commonwealth Serum Laboratory (CSL) and Lelystad (LEL) PPDs in cattle upon experimental infection with M. bovis. (a) Comparison of IFN-γ responses to PPDs in control (n = 5) as well as M. bovis Ravenel- (n = 5), and M. bovis 95-1315-infected (n = 5) animals pooled over time. (b) Comparison of IFN-γ responses to PPDs in control (n = 7) as well as M. bovis 95-1315- (n = 8), and M. bovis 10-7428-infected (n = 8) animals pooled over time. PPDa refers to M. avium-derived PPD, PPDb refers to M. bovis-derived PPD, and B-A indicates values for PPDb minus PPDa. Asterisks indicate that responses differed (*, P < 0.05; **, P < 0.01).
Article Snippet: PPDs and peptide cocktails were also used for comparison of a
Techniques: Infection, Derivative Assay
Journal: Clinical and Vaccine Immunology : CVI
Article Title: Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs
doi: 10.1128/CVI.00519-13
Figure Lengend Snippet: Evaluation of IFN-γ responses in samples obtained from dairy cattle within a tuberculosis-affected herd (i.e., natural infection). Comparison of CSL (Commonwealth Serum Laboratories) and Lelystad (Prionics AG) M. avium-derived PPD (PPDa) and M. bovis-derived PPD (PPDb). Responses from M. bovis-infected (n = 56) animals from a tuberculosis-affected dairy in Colorado. Animals were categorized as infected based on positive culture of M. bovis (infected, n = 56). **, responses to Lelystad PPDs exceeded (P < 0.01) respective responses to CSL PPDs.
Article Snippet: PPDs and peptide cocktails were also used for comparison of a
Techniques: Infection, Derivative Assay
Journal: Clinical and Vaccine Immunology : CVI
Article Title: Clinical and Diagnostic Developments of a Gamma Interferon Release Assay for Use in Bovine Tuberculosis Control Programs
doi: 10.1128/CVI.00519-13
Figure Lengend Snippet: Comparison of current (B1G) and new (B2G) Bovigam (Prionics AG) kits using responses to individual antigens and PPDb minus PPDa with samples from control (n = 7) as well as M. bovis 95-1315- (n = 8), and M. bovis 10-7428-infected (n = 8) animals. (a) IFN-γ responses pooled over time, (b) responses to prechallenge (Commonwealth Serum Laboratories) and Lelystad (Prionics AG) PPDb minus PPDa, (c) responses to 4-week-postchallenge CSL and Lelystad PPDb minus PPDa, and (d) responses to 12-week-postchallenge CSL and Lelystad PPDb minus PPDa.
Article Snippet: PPDs and peptide cocktails were also used for comparison of a
Techniques: Infection